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pcmv dr8 2 dvpr  (Addgene inc)


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    Addgene inc pcmv dr8 2 dvpr
    Pcmv Dr8 2 Dvpr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-dr8%2E2/pCMV-dR8%2E2+dvpr+(Plasmid+%238455)/pmc12907852-54-32-34
    Average 96 stars, based on 885 article reviews
    pcmv dr8 2 dvpr - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Plasmid Preparation:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Transfection:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    shRNA:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Recombinant:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Selection:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Labeling:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Real-time Polymerase Chain Reaction:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    RNA Sequencing Assay:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).

    Sequencing:

    Article Title: Cezanne is a critical regulator of pathological arterial remodelling by targeting β-catenin signalling
    Article Snippet: 128 Briefly, 293T cells were transfected with the lentiviral vector and the 129 packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from 130 Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific 131 Inc) according to the manufacturer's instructions.

    Article Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice
    Article Snippet: Briefly, 293T cells were transfected with the abovementioned shRNA plasmids and the packaging plasmids, pCMV-dR8.2 and pCMV-VSV-G (both obtained from Addgene), using TurboFect Transfection Reagent (Thermo Fisher Scientific, Inc) according to the manufacturer’s instructions.

    Article Title: Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress control.
    Article Snippet: Article Viral hijacking of hnRNPH1 unveils a G-quadruplex- driven mechanism of stress control

    Article Title: The role of Nef in the long-term persistence of the replication-competent HIV reservoir in South African women.
    Article Snippet: Plasmid pNL4.3 ΔGag/Pol eGFP (58, 59) containing the participantspecific nef gene, pCMV-DR8.2 (encoding Gag/Pol; Addgene catalog number: 12263), and pMD2.G (encoding VSV-G; Addgene catalog number: 12259) were co-transfected at a ratio of 0.4:1:1, respectively, as previously described (34).


    Article Title: Engineered Herpes Simplex Virus-1 (HSV-1) vectors and uses thereof
    Article Snippet: Three hours later the ̃80% confluent cells were co-transfected with the pLV-hEF1a-attP_BxB 1-EYFP-2A-Hygro vector, the packaging plasmid pCMV-dR8.2 (Addgene) and the envelope plasmid pCMV-VSV-G (Addgene), as described previously (1) using Attractene reagent (Qiagen) by following manufacturer's protocol.

    Article Title: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability
    Article Snippet: For lentiviral particles production, HEK293T cells were co-transfected with the lentiviral vector containing HA-tagged ZXDA (pTRIPZ_HA-ZXDA) along with pCMV-dR8.2 (Addgene, #8455) and pCMV-VSV-G (Addgene, #8454).



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